Publications

Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T

Bosma, Elleke F.; Koehorst, Jasper J.; van Hijum, Sacha A.F.T.; Renckens, Bernadet; Vriesendorp, Bastienne; van de Weijer, Tom; Schaap, Peter J.; de Vos, Willem M.; van der Oost, John; van Kranenburg, Richard

Summary

Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216^T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.